5K1G
Crystal structure of reduced Prx3 from Vibrio vulnificus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100.15 |
Detector technology | CCD |
Collection date | 2015-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 73.871, 73.871, 62.254 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.987 - 1.899 |
R-factor | 0.224 |
Rwork | 0.220 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f82 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.574 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.899 | 1.900 |
Rmerge | 0.058 | |
Number of reflections | 15873 | |
<I/σ(I)> | 4.57 | |
Completeness [%] | 99.3 | |
Redundancy | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287.15 | 0.8 M sodium acetate, 0.1 M Tris-HCl, 0.2M sodium chloride |