5K19

Crystal structure of WD repeat-containing protein 20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 31
Unit cell lengths	120.014, 120.014, 95.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.626 - 2.602
R-factor	0.1939
Rwork	0.191
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1erj
RMSD bond length	0.009
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.089	0.898
Number of reflections	46508
<I/σ(I)>	19.8	1.5
Completeness [%]	98.8	99.1
Redundancy	2.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M sodium citrate, 7% PEG 3350, 0.15 M NaCl