5K15
Crystal structure of laccase from Thermus thermophilus HB27 (Cu2PO, 8 min)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-03 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.440, 109.860, 96.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.632 - 1.550 |
| R-factor | 0.1933 |
| Rwork | 0.191 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.632 | 1.700 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.083 | 0.520 |
| Number of reflections | 55015 | |
| <I/σ(I)> | 13.74 | 2.68 |
| Completeness [%] | 97.8 | 99.4 |
| Redundancy | 4.37 | 4.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M HEPES, pH 7.5, 70% MPD |
