5JZL
The Structure of Monomeric Ultra Stable Green Fluorescent Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.352, 95.706, 59.649 |
| Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
| Resolution | 57.820 - 1.800 |
| R-factor | 0.17675 |
| Rwork | 0.175 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jzk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.727 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.136 | 0.869 |
| Number of reflections | 47783 | |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 99.7 | 95.3 |
| Redundancy | 7.6 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 22% w/v PEG 8000, 0.2 M sodium chloride, 0.1 M phosphate-citrate buffer pH 3.7, 4% v/v acetone |
