5JZ7
NGF IN COMPLEX WITH MEDI578 scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | I 4 |
| Unit cell lengths | 182.085, 182.085, 109.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.020 - 3.400 |
| R-factor | 0.2582 |
| Rwork | 0.258 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1btg 1aqk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5 PACIOREK) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.020 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.370 | 1.100 |
| Number of reflections | 24799 | |
| <I/σ(I)> | 5.3 | 1.9 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18 % PEG 8000, 150 mM NaCl, 100 mM MES pH 6.5 |
