5JYF

Structures of Streptococcus agalactiae GBS GAPDH in different enzymatic states

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	79.215, 112.634, 147.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.550 - 2.620
R-factor	0.23359
Rwork	0.231
R-free	0.27836
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4QX6_A
RMSD bond length	0.007
RMSD bond angle	1.239
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	147.630	2.760
High resolution limit [Å]	2.620	2.620
Rmerge	0.086	0.840
Number of reflections	40180
<I/σ(I)>	9.7	1.1
Completeness [%]	99.5	99.9
Redundancy	4.1	4.1
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20-28% PEG4000, 0.1M Mes