5JXT

Crystal structure of MtISWI bound with histone H4 tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	102.240, 119.316, 132.495
Unit cell angles	89.59, 105.96, 93.08

Refinement procedure

Resolution	42.000 - 3.009
R-factor	0.221
Rwork	0.218
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.589
Data reduction software	HKL-2000 (v705a)
Data scaling software	HKL-2000 (v705a)
Phasing software	PHASER (v7)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.000
High resolution limit [Å]	3.000
Number of reflections	112290
<I/σ(I)>	7.2
Completeness [%]	94.2
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	Polyethylene glycol 400