5JX5

GH6 Orpinomyces sp. Y102 enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	309.859, 87.353, 82.551
Unit cell angles	90.00, 93.95, 90.00

Refinement procedure

Resolution	27.760 - 1.800
R-factor	0.2106
Rwork	0.209
R-free	0.23920
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.044	0.025	0.359
Number of reflections	200111
<I/σ(I)>	20.9
Completeness [%]	98.2	99	86.3
Redundancy	3.2	3.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% PEG 6K, 0.1M ammonium phosphate buffer, 2% MPD