5JX2
Crystal structure of MglB-2 (Tp0684) from Treponema pallidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.556, 130.025, 88.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.785 - 2.050 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22550 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.735 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.075 | |
| Number of reflections | 28333 | |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M BIS-TRIS pH 6.5 20% PEG MME 5000 |
