Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.450, 46.260, 48.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.432 - 1.439 |
| R-factor | 0.1879 |
| Rwork | 0.187 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | modified 2HI2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.679 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2299: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.432 |
| High resolution limit [Å] | 1.439 |
| Number of reflections | 18088 |
| <I/σ(I)> | 21.6 |
| Completeness [%] | 98.7 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES PH 6.0, 34 % (W/V) PEG4000, 100 mM AMMONIUM SULPHATE |
