5JW7

Crystal structure of SopA-Trim56 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.99987
Spacegroup name	P 31 1 2
Unit cell lengths	71.015, 71.015, 122.944
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.477 - 2.849
R-factor	0.2261
Rwork	0.223
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qza 2ct2
RMSD bond length	0.004
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.500	2.950
High resolution limit [Å]	2.849	2.849
Rmerge	0.074	0.835
Number of reflections	8375
<I/σ(I)>	31.15	3.64
Completeness [%]	98.4	84.05
Redundancy	20	3.64

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	28% w/v Polyacrylate 2100 sodium salt, 0.2M NaCl, 0.1M MES pH 6