5JVV
Crystal structure and characterization an elongating GH family 16 beta-1,3-glucosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.282, 91.469, 59.532 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 37.167 - 1.589 |
| R-factor | 0.1496 |
| Rwork | 0.149 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wdt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.121 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.170 | 1.650 |
| High resolution limit [Å] | 1.589 | 1.589 |
| Rmerge | 0.090 | 0.303 |
| Number of reflections | 62905 | |
| <I/σ(I)> | 17.93 | 6.11 |
| Completeness [%] | 96.6 | 94.1 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 292 | 35% PEG 3350, 0.1M Na-Citrate pH4.4 |
