5JVR

The neck-linker of Mus musculus KIF3A fused to the alpha 7 helix of Drosophila melanogaster Kinesin-1 fused to EB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	53.172, 80.189, 155.375
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.880 - 2.100
R-factor	0.198
Rwork	0.196
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yib
RMSD bond length	0.001
RMSD bond angle	0.328
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.880	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.089	0.571
Number of reflections	52346
<I/σ(I)>	31.1	3.2
Completeness [%]	100.0	99.1
Redundancy	13.2	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	32% (w/v) polyethylene glycol dimethyl ether (DMPEG) 500, 30 mM MgCl2, 10 mM diethylenetriamine, 100 mM sodium 3-[4-(2-Hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPS) pH 8.5