5JVE
L16I mutant of cytochrome c prime from Alcaligenes xylosoxidans: Ferrous state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.520, 53.520, 179.682 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.120 |
| Rwork | 0.153 |
| R-free | 0.18100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.015 |
| RMSD bond angle | 325.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | SHELXDE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.064 | 0.652 |
| Number of reflections | 61939 | |
| <I/σ(I)> | 9.6 | 1.5 |
| Completeness [%] | 98.7 | 94.8 |
| Redundancy | 5.5 | 4.5 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2M Ammonium sulfate, 0.1 M HEPES pH 7.5 |
