5JV4
Structure of F420 binding protein, MSMEG_6526, from Mycobacterium smegmatis with F420 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9501 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.951, 84.241, 75.980 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 75.970 - 1.700 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23081 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zky |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.831 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.970 | 9.310 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.122 | |
| Number of reflections | 96899 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Sodium Acetate pH 4.6 20 mM Calcium Chloride 28% MPD |
