5JUA
Nitric oxide complex of the L16I mutant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.897, 53.897, 180.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.130 |
| Rwork | 0.152 |
| R-free | 0.18680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.014 |
| RMSD bond angle | 294.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELXDE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.190 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.030 | 0.599 |
| Number of reflections | 57236 | |
| <I/σ(I)> | 24.2 | 3.1 |
| Completeness [%] | 97.4 | 93.8 |
| Redundancy | 6.2 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2 M Ammonium sulfate, 0.1M HEPES pH 7.5 |
