5JSL
The L16F mutant of cytochrome c prime from Alcaligenes xylosoxidans: Ferrous form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.273, 53.273, 180.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.250 |
| Rwork | 0.146 |
| R-free | 0.17920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.014 |
| RMSD bond angle | 315.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | SHELXDE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.059 | 0.684 |
| Number of reflections | 43297 | |
| <I/σ(I)> | 11.4 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.2 | 5.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5 |
