5JS9
Uncleaved prefusion optimized gp140 trimer with an engineered 8-residue HR1 turn bound to broadly neutralizing antibodies 8ANC195 and PGT128
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 261.955, 261.955, 261.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.826 - 6.918 |
| R-factor | 0.2878 |
| Rwork | 0.284 |
| R-free | 0.32170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c7k |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.150 |
| High resolution limit [Å] | 6.900 | 6.900 |
| Number of reflections | 5022 | |
| <I/σ(I)> | 15.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.1 | 20.3 |
| CC(1/2) | 0.330 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 0.05 M lithium sulfate, 0.05 M sodium sulfate, 20% w/v PEG400, 0.05 M Tris-HCl, pH 8.7 |
