5JRV

Crystal structure of Fe(II) NO-bound H-NOX protein from C. subterraneus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2015-02-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.999919
Spacegroup name	P 21 21 2
Unit cell lengths	80.385, 127.626, 42.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.979 - 1.953
R-factor	0.2031
Rwork	0.202
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u55
RMSD bond length	0.004
RMSD bond angle	0.780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.093	0.434
Number of reflections	32785
<I/σ(I)>	14.9	3.67
Completeness [%]	99.9	100
Redundancy	6.8
CC(1/2)	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Anaerobically grown under Argon. 20 mg/mL protein with well condition of 0.15 M NaI, 25% PEG3350.