5JRJ

Crystal Structure of Herbaspirillum seropedicae RecA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-15
Detector	BRUKER SMART 6000
Wavelength(s)	1.54
Spacegroup name	P 61
Unit cell lengths	87.410, 87.410, 91.353
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.578 - 1.700
R-factor	0.1772
Rwork	0.175
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xmv
RMSD bond length	0.015
RMSD bond angle	1.621
Data reduction software	PROTEUM PLUS (Version 2010.5)
Data scaling software	PROTEUM PLUS (Version 2010.5)
Phasing software	PHASER (Phaser-2.6.0)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.578	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.047	0.628
Number of reflections	42574
<I/σ(I)>	31.03	2.08
Completeness [%]	97.7	79.5
Redundancy	5.18	1.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293.15	Single crystals were obtained with 0.25 M CaCl2 and 14% w/v PEG 3350 with addition of Polypropylene glycol P 400 (PPG 400) onto the drop to give a final concentration of 5-8% w/v.