5JRG

Crystal structure of the nucleosome containing the DNA with tetrahydrofuran (THF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98000
Spacegroup name	P 21 21 21
Unit cell lengths	99.491, 108.773, 169.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.146 - 2.500
R-factor	0.1956
Rwork	0.193
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.012
RMSD bond angle	1.279
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.093	0.060	0.418
Number of reflections	64545
<I/σ(I)>	6.5
Completeness [%]	99.8	99.8	99.8
Redundancy	9.2	11.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride