5JRC
Crystal structure of NeC3PO in complex with ssRNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.416, 77.648, 87.754 |
| Unit cell angles | 90.00, 118.81, 90.00 |
Refinement procedure
| Resolution | 29.070 - 1.900 |
| R-factor | 0.20688 |
| Rwork | 0.206 |
| R-free | 0.22983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 71365 |
| <I/σ(I)> | 16.3 |
| Completeness [%] | 96.8 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 10% (w/v) PEG 8000, 100 mM imidazole/HCl pH 8.0, 200 mM Ca(OAc)2 |
