5JR9
Crystal structure of apo-NeC3PO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.438, 92.712, 92.745 |
| Unit cell angles | 114.29, 113.39, 95.76 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.2298 |
| Rwork | 0.229 |
| R-free | 0.25157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.358 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 80578 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 95.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 5% v/v tacsimate (pH 7.0), 0.1 M HEPES (pH 7.0), 10% w/v PEG monomethyl ether 5,000 |
