5JR4
Crystal structure of FimH A27V/V163A from E. coli UTI89 bound to FimG N-terminal extension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-03-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.320, 33.030, 72.450 |
| Unit cell angles | 90.00, 120.62, 90.00 |
Refinement procedure
| Resolution | 62.348 - 2.596 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1klf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.658 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.350 | 2.730 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.164 | 0.482 |
| Number of reflections | 7964 | |
| <I/σ(I)> | 8.1 | 3.3 |
| Completeness [%] | 99.3 | 95.1 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M MES pH 5.6, 0.2 M Calcium Acetate, 5% PEG 8000 |
