5JR1

Crystal structure of 10E8 gHV-matureL antigen-binding fragment.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	68.784, 77.863, 85.003
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.306 - 1.601
R-factor	0.186
Rwork	0.185
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g6f
RMSD bond length	0.010
RMSD bond angle	1.075
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	51383
<I/σ(I)>	9.83
Completeness [%]	84.6
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	21% PEG 8000, 0.17M ZnAc, MES, pH6.0