5JQN
NitN Amidase from Neterenkonia sp. AN1 after thrombin His-tag removal.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.423, 115.794, 65.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.566 - 1.190 |
| R-factor | 0.1753 |
| Rwork | 0.175 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hkx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.566 | |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmeas | 0.037 | |
| Total number of observations | 580951 | |
| Number of reflections | 91215 | |
| <I/σ(I)> | 25.13 | |
| Completeness [%] | 98.6 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.15 | 2M Ammonium Sufate |
