5JQI
Crystal structure of FimH A62S from E. coli UTI89 bound to FimG N-terminal extension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-02-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.440, 75.560, 78.440 |
| Unit cell angles | 68.35, 69.00, 77.43 |
Refinement procedure
| Resolution | 48.666 - 1.962 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jwn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.790 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.141 | 0.706 |
| Number of reflections | 75411 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 94.2 | 90.4 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Calcium Acetate, 0.1 M HEPES 7.5, 10% PEG 8000 |
