5JQA
CaM:RM20 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.016, 57.048, 45.068 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 28.720 - 1.800 |
| R-factor | 0.15515 |
| Rwork | 0.153 |
| R-free | 0.18845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5g |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.357 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 15660 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 95.5 | |
| Redundancy | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium acetate, pH 4.6, 25% w/v PEG4000 |
