5JPZ

Crystal structure of HAT domain of human Squamous Cell Carcinoma Antigen Recognized By T Cells 3, SART3 (TIP110)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-22
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	0.973720
Spacegroup name	P 21 21 21
Unit cell lengths	105.568, 118.351, 133.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.356 - 3.045
R-factor	0.2383
Rwork	0.236
R-free	0.28010
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.311
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.180	59.180	3.210
High resolution limit [Å]	3.040	9.630	3.040
Rmerge	0.083	0.025	0.842
Rmeas	0.093
Rpim	0.042
Total number of observations	155195
Number of reflections	32447
<I/σ(I)>	12.5	45	1.7
Completeness [%]	99.3	99.1	96.6
Redundancy	4.8	4.2	4.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283.15	0.1M Monosaccharides; 0.1M Tris/Bicine (pH 7.3), 32%PEG550MME_PEG20K