5JPO

Complex structure of human elongation factor 1B gamma GST-liked domain and delta N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	293.15
Detector technology	CCD
Collection date	2016-03-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	115.499, 101.054, 93.732
Unit cell angles	90.00, 98.75, 90.00

Refinement procedure

Resolution	44.358 - 1.998
R-factor	0.1693
Rwork	0.168
R-free	0.20260
RMSD bond length	0.007
RMSD bond angle	1.057
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	1.998	4.310	2.000
Rmerge	0.105	0.054	0.708
Number of reflections	71801
<I/σ(I)>	9
Completeness [%]	99.9	99.9	99.2
Redundancy	7.4	7.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	SOKALAN CP7, KCl, HEPES