5JPJ
Crystal structure of 6aJL2-R24G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18076 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 69.929, 77.796, 93.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.694 - 2.000 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mkw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.773 | 2.001 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.566 |
| Number of reflections | 17490 | |
| <I/σ(I)> | 14.8 | 2.3 |
| Completeness [%] | 98.7 | 91.5 |
| Redundancy | 4.6 | 3.7 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1 M sodium/potassium tartrate, 100 mM Tris-HCl, pH 7.0, 200 mM lithium sulfate |
