5JPG
Rat Galectin 5 with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.024, 65.763, 112.718 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.024 - 1.900 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nv1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.024 | 1.968 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.252 |
| Number of reflections | 23584 | |
| <I/σ(I)> | 7.6 | 2.74 |
| Completeness [%] | 99.8 | 99.74 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 10% PEG 8000, 100 mM Tris pH 7.0, 200 mM MgCl2 |
