5JP6
Bdellovibrio bacteriovorus peptidoglycan deacetylase Bd3279
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 61 |
| Unit cell lengths | 112.434, 112.434, 79.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.370 - 1.500 |
| R-factor | 0.1458 |
| Rwork | 0.145 |
| R-free | 0.16680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c1g |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 97.370 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 91430 |
| <I/σ(I)> | 25.1 |
| Completeness [%] | 99.9 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Na cacodylate pH 6.5 0.2M Mg acetate 20% w/v/ PEG 8000 |
