5JP3
Structure of Xanthomonas campestris effector protein XopD bound to ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.748, 132.281, 117.310 |
| Unit cell angles | 90.00, 105.84, 90.00 |
Refinement procedure
| Resolution | 43.297 - 2.900 |
| R-factor | 0.2431 |
| Rwork | 0.241 |
| R-free | 0.28510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiv 1ubq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.128 | 0.568 |
| Number of reflections | 37920 | |
| <I/σ(I)> | 8.1 | 2.4 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M CHES (pH 9.5) 1.0M sodium citrate |
