5JOZ
Bacteroides ovatus Xyloglucan PUL GH43B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.460, 71.400, 160.300 |
| Unit cell angles | 90.00, 94.78, 90.00 |
Refinement procedure
| Resolution | 52.190 - 2.280 |
| R-factor | 0.19513 |
| Rwork | 0.192 |
| R-free | 0.25023 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.190 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.092 | 0.624 |
| Number of reflections | 46976 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 0.2 M sodium acetate pH 5, 20-30% PEG-3350 |
