5JOU
Bacteroides ovatus Xyloglucan PUL GH31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.892, 109.020, 145.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.350 - 1.500 |
| R-factor | 0.16123 |
| Rwork | 0.160 |
| R-free | 0.18354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xvg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.350 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.124 | 1.090 |
| Number of reflections | 187821 | |
| <I/σ(I)> | 12.4 | 1 |
| Completeness [%] | 96.7 | 74.5 |
| Redundancy | 7.5 | 4.2 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M potassium thiocyanate, 14-20% (w/v) PEG-3350 |
