5JOQ

Crystal Structure of an ABC Transporter Substrate-Binding Protein from Listeria monocytogenes EGD-e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 63 2 2
Unit cell lengths	118.380, 118.380, 106.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.410 - 1.990
R-factor	0.152
Rwork	0.151
R-free	0.17100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q8q
RMSD bond length	0.014
RMSD bond angle	1.002
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX ((DEV_2203))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.410	2.040
High resolution limit [Å]	1.990	1.990
Rmerge	0.091	0.662
Number of reflections	30557
<I/σ(I)>	21.7	4.3
Completeness [%]	99.9	99.7
Redundancy	12	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2M NH4 Citrate pH 7, 0.1M Bis-Tris Propane pH 7