5JOC
Crystal structure of the S61A mutant of AmpC BER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 63 |
| Unit cell lengths | 207.208, 207.208, 45.635 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.17799 |
| Rwork | 0.177 |
| R-free | 0.20015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f1g |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.118 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 109761 | |
| <I/σ(I)> | 29.8 | |
| Completeness [%] | 96.5 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 25% PEG 3350, 0.1 M Citric Acid, NaOH pH 3.5 |
