5JO8
CEP104 TOG domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0, 0.96 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 48.360, 57.220, 102.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.772 - 1.400 |
| R-factor | 0.2192 |
| Rwork | 0.219 |
| R-free | 0.23830 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.065 | 2.760 |
| Number of reflections | 55668 | |
| <I/σ(I)> | 19.02 | 1.05 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 16.4 | 16.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 25% PEG 1500, 0.1 M MMT pH 5.0 |
