5JNY
Crystal Structure of 10E8 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2011-07-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 248.627, 248.627, 54.256 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.836 - 3.041 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.717 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.160 |
High resolution limit [Å] | 3.050 | 6.570 | 3.050 |
Rmerge | 0.221 | 0.094 | 0.684 |
Rmeas | 0.232 | ||
Rpim | 0.070 | ||
Total number of observations | 397174 | ||
Number of reflections | 37419 | ||
<I/σ(I)> | 3.5 | ||
Completeness [%] | 99.7 | 99.1 | 98.3 |
Redundancy | 10.6 | 10.8 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 6% PEG 8000, 25% Isopropanol, 0.1 M Imidazole pH 6.5 |