5JNP

Crystal structure of a rice (Oryza Sativa) cellulose synthase plant conserved region (P-CR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794
Spacegroup name	F 2 3
Unit cell lengths	150.817, 150.817, 150.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.473 - 2.404
R-factor	0.2434
Rwork	0.240
R-free	0.27150
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.244
Data reduction software	HKL-2000 (710)
Data scaling software	HKL-2000 (710)
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.087	0.068
Rmeas	0.088
Rpim	0.015
Total number of observations	505517
Number of reflections	11173
<I/σ(I)>	11.8
Completeness [%]	99.9	99.1	100
Redundancy	45.2	42.6	45.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.6	277	1.0 M trisodium citrate dihydrate