5JMT
Crystal structure of Zika virus NS3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.378, 68.990, 57.903 |
| Unit cell angles | 90.00, 94.39, 90.00 |
Refinement procedure
| Resolution | 44.276 - 1.796 |
| R-factor | 0.1813 |
| Rwork | 0.180 |
| R-free | 0.19960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.777 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.276 | 1.830 |
| High resolution limit [Å] | 1.796 | 1.800 |
| Number of reflections | 37570 | |
| <I/σ(I)> | 22.7 | 3 |
| Completeness [%] | 96.2 | 69.5 |
| Redundancy | 6.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.2 | 291 | 0.1 M Sodium citrate tribasic dihydrate (pH 5.5), 22%(w/v) polyethylene glycol 1000 |
