5JMF
Heparinase III-BT4657 gene product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.690, 45.741, 80.514 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 20.503 - 1.854 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.20320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.503 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 58055 |
| <I/σ(I)> | 27.8 |
| Completeness [%] | 98.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 20% PEG 3350, 100mM Bis-Tris pH 5.5, 200mM Magnesium chloride,500mM NDSB-201 as additive inn crystallization drop |
