5JLU
Crystal Structure of Adhesin competence repressor (AdcR) from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 105.460, 105.460, 36.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.360 - 2.200 |
| R-factor | 0.20358 |
| Rwork | 0.201 |
| R-free | 0.24241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jls |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.983 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.668 | |
| Number of reflections | 10996 | |
| <I/σ(I)> | 17.8 | 3.3 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 11.5 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 295 | 0.1 M sodium/phosphate phosphate, pH 7.2, 30% PEG200 |
