5JLS

Crystal Structure of Adhesin competence repressor (AdcR) from Streptococcus pyogenes (C-terminally His tagged)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 42 21 2
Unit cell lengths	105.560, 105.560, 36.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.20429
Rwork	0.200
R-free	0.27821
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.844
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200
High resolution limit [Å]	2.700	2.700
Number of reflections	6084
<I/σ(I)>	21
Completeness [%]	99.5
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.1 M imidazole, pH 7.0, 10% PEG3350, 5 mM magnesium chloride, 5 mM nickel chloride, 5 mM cobalt chloride, 14% PEG400 as additive