5JLI
Nitric oxide complex of the L16A mutant of cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.629, 53.629, 180.523 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.440 - 1.550 |
| R-factor | 0.1728 |
| Rwork | 0.171 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yl0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.184 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.073 | 0.760 |
| Number of reflections | 23047 | |
| <I/σ(I)> | 11.9 | 1.9 |
| Completeness [%] | 98.8 | 98.1 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 2.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5 |
