5JKN
Crystal structure of deubiquitinase MINDY-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.93927 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 99.671, 99.671, 165.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.670 - 3.000 |
| R-factor | 0.19907 |
| Rwork | 0.197 |
| R-free | 0.24231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Partial model obatined from experimental phasing |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.670 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.065 | 0.880 |
| Number of reflections | 17325 | |
| <I/σ(I)> | 14.4 | 2.1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 100mM Hepes-Na pH 7.5, 200mM NaCl and 25% PEG4000 |
