5JJX
Crystal structure of the HAT domain of sart3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 63 |
| Unit cell lengths | 135.113, 135.113, 43.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2077 |
| Rwork | 0.207 |
| R-free | 0.23930 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.067 | 0.032 | 0.968 |
| Rmeas | 0.069 | ||
| Rpim | 0.025 | ||
| Total number of observations | 256972 | ||
| Number of reflections | 30852 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 8.3 | 8.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 30% PEG 3350, 0.2 M NaCl, 0.1 M HEPES pH7.5 |
