5JJT
Crystal structure of a type 5 serine/threonine protein phosphatase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.408, 102.705, 95.290 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 45.797 - 2.103 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wao |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.705 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.091 | |
| Number of reflections | 55086 | |
| <I/σ(I)> | 12.97 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 289 | 0.1M HEPES pH7.2, 22.5% PEG 3350, 20% glycol |
