5JJL

Rho transcription termination factor bound to rU8 and 5 ADP-BeF3 molecules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	69.091, 199.071, 111.666
Unit cell angles	90.00, 105.02, 90.00

Refinement procedure

Resolution	49.520 - 3.200
R-factor	0.234
Rwork	0.232
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jji
RMSD bond length	0.006
RMSD bond angle	0.573
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	3.310
High resolution limit [Å]	3.200	3.200
Rmerge		0.580
Number of reflections	46060
<I/σ(I)>		1.8
Completeness [%]	96.1	96.4
Redundancy		2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	200 mM KOAc, 40% MPD, 0.5% ethyl acetate